Anti-Protein A antibody (HRP) (ab18596)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES. pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgY
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab18596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
ELISA 1/5000 - 1/20000.

Target

  • RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

References for Anti-Protein A antibody (HRP) (ab18596)

ab18596 has not yet been referenced specifically in any publications.

Product Wall

It appears that you are asking if a capture(sandwich) ELISA was performed.

We do not run a capture test on the the conjugate, only a direct ELISA to determine the functionality of the conjugation.

We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (http://www.abcam.com/index.html?page...

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Thank you for contacting us.

Worldwide, we have 16 vials of this antibody in stock. We do not have more as it is not a bestseller, but sells quite solid. This product is not a niche product either, therefore, we constantly have have a certain ...

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Thank you for contacting us.

Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

For ab19483, chicken polyclonal t...

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Thank you for contacting us.

We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vial...

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Thank you for your enquiry and your interest in our products.

I have checked the stock viability and can confirm the followings:

- ab19483 - several vials from the same bleed and purification;

- ab18596 - only one vial lef...

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Thank you for contacting us. I apologize for being late in getting back to you.

I can confirm that both antibodies ab18596 and ab19483 have similar IgY isotype.

I hope this information is helpful to you. Please do not hesitate to cont...

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Thank you for confirming this information and for your help and cooperation with this case.

As a special exception as the customer has used so many vials succesfully, we can extend the guarantee period in this case and Iasked our accounting ...

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Thank you for your reply.

I appreciate your cooperation and understand your concerns. It is regrettable the results from lot 793467have not been assatisfactory for the customer. I can confirm that ab18596 and ab18596-1 are the same antibody....

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As this is a polyclonal antibody, some batch variability could be expected. It is possible there could also be variabilitywith the detection kit or secondary antibodyin this case.

Could you confirm if the customer is satisfied with the resul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"