Anti-Protein A antibody [SPA-27] (Biotin) (ab49734)

Overview

  • Product nameAnti-Protein A antibody [SPA-27] (Biotin)
    See all Protein A primary antibodies
  • Description
    Mouse monoclonal [SPA-27] to Protein A (Biotin)
  • ConjugationBiotin
  • SpecificitySpecific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. The activity of this antibody is due to specific antigen antibody interaction with protein A; Protein A reacts minimally with the Fc portion of this mouse IgG1 antibody. The antibody will bind to protein A even in the presence of IgG.
  • Tested applicationsSuitable for: ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Purified Protein A from Cowan I strain of Staphy­lococcus aureus.

  • General notes

    The immunoglobulin fraction of the ascites fluid is conjugated to epsilon amino caproyl biotin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesImmunoglobulin Fraction of Mouse Ascites Fluid
  • ClonalityMonoclonal
  • Clone numberSPA-27
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/80000.
Dot blot 1/5000.

Target

  • RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.
  • Database links
    • Alternative names
      • gG-binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody
      • Staphylococcal protein A antibody

    References for Anti-Protein A antibody [SPA-27] (Biotin) (ab49734)

    ab49734 has not yet been referenced specifically in any publications.

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