SpecificitySpecific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. The activity of this antibody is due to specific antigen antibody interaction with protein A; Protein A reacts minimally with the Fc portion of this mouse IgG1 antibody. The antibody will bind to protein A even in the presence of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence.
Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.