Overview

  • Product name
    Protein Block
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ELISAmore details
  • General notes

    Protein Block has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. Protein Block has been shown to be effective for immunohistochemical, ELISA, blot and In-situ techniques and requires no mixing or diluting.

    Reagent: 60 ml

    Procedure:

    Immunohistochemistry:

    Incubate tissue section for 5 minutes at either room temperature or 37°C prior to application of the primary antibody. After incubation, rinse once in buffer (Optional) (NOTE: Do not incubate tissue sections in excess of 10 minutes or a reduction in desired staining may occur).

    For bulk staining, pour Protein Block in a covered staining tray and dip slides for 5 minutes. Replace with fresh Protein Block after 5-10 uses. This step can be performed at the time of deparaffinization.

    For antibodies with particularly high background staining, dilute Protein Block in PBS (1/5 - 1/10) and use as a wash buffer in addition to the blocking step.

    ELISA:

    Add Protein Block to microtiter well and incubate for 2-10 minutes prior to addition of sample. Rinse, and continue procedure. (NOTE: Do not incubate in excess of 10 minutes).

    Chemiluminescent Blot:

    Protein Block has been reported to be an effective blocker for this technique with incubation times of one hour at room temperature or overnight at 4°C.

    IHC Protocol Summary

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C.
  • Storage buffer
    Constituents: 1% Monobasic dihydrogen potassium phosphate, 0.014% Dibasic monohydrogen sodium phosphate, 1% Triton-X-100, 1% Gelatin, 0.002% Potassium chloride, 1% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

References

ab156024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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