Anti-Protein G antibody (Biotin) (ab7251)

Overview

  • Product nameAnti-Protein G antibody (Biotin)
    See all Protein G primary antibodies
  • Description
    Rabbit polyclonal to Protein G (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Full length native protein (purified) corresponding to Protein G. Strepococcus species

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab7251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationCell Wall and Secreted
  • Alternative names
    • IgG binding protein G antibody
    • IgG-binding protein G antibody
    • Immunoglobulin G binding protein G [Precursor] antibody
    • spg antibody
    • spg immunoglobulin G-binding protein antibody
    • SPG2_STRSG antibody
    see all

References for Anti-Protein G antibody (Biotin) (ab7251)

ab7251 has not yet been referenced specifically in any publications.

Product Wall

The answer is no, it is not epitope mapped. The antibody is polyclonal and raised against the whole protein. It regonizes multiple epitopes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"