Anti-Protein G antibody (Biotin) (ab7251)

Overview

  • Product name
    Anti-Protein G antibody (Biotin)
    See all Protein G primary antibodies
  • Description
    Rabbit polyclonal to Protein G (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Full length native protein (purified) corresponding to Protein G. Strepococcus species

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Cell Wall and Secreted
  • Alternative names
    • IgG binding protein G antibody
    • IgG-binding protein G antibody
    • Immunoglobulin G binding protein G [Precursor] antibody
    • spg antibody
    • spg immunoglobulin G-binding protein antibody
    • SPG2_STRSG antibody
    see all

References

ab7251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The answer is no, it is not epitope mapped. The antibody is polyclonal and raised against the whole protein. It regonizes multiple epitopes.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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