Anti-Protein G antibody (FITC) (ab7249)

Overview

  • Product nameAnti-Protein G antibody (FITC)
    See all Protein G primary antibodies
  • Description
    Rabbit polyclonal to Protein G (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityNo reaction is observed against Protein A.
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cytmore details
  • Immunogen

    Protein G [Streptococcus sp]

  • General notes

    Diaminotriazynlaminofluorescein (DTAF) (Molecular Weight 530 daltons), Absorption Wavelength: 495 nm, Emission Wavelength: 528 nm, Fluorochrome/Protein Ratio: 4.0 moles DTAF per mole of Rabbit IgG

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab7249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
  • Application notesImmunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays.
  • Target

    • RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
    • Cellular localizationCell Wall and Secreted
    • Alternative names
      • IgG binding protein G antibody
      • IgG-binding protein G antibody
      • Immunoglobulin G binding protein G [Precursor] antibody
      • spg antibody
      • spg immunoglobulin G-binding protein antibody
      • SPG2_STRSG antibody
      see all

    References for Anti-Protein G antibody (FITC) (ab7249)

    ab7249 has not yet been referenced specifically in any publications.

    Product Wall

    I was informed that unfortunately our Protein G products were not tested on denatured HC or LC individully. I'm sorry I cannot help you more on this occasion,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"