Overview

  • Product nameAnti-Protein G antibody (HRP)
    See all Protein G primary antibodies
  • Description
    Chicken polyclonal to Protein G (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Purified recombinant protein G lacking the albumin binding region.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES. pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAfter multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein G agarose and conjugated with horseradish peroxidase (HRP).
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
ELISA 1/5000 - 1/20000.

Target

  • RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationCell Wall and Secreted
  • Alternative names
    • IgG binding protein G antibody
    • IgG-binding protein G antibody
    • Immunoglobulin G binding protein G [Precursor] antibody
    • spg antibody
    • spg immunoglobulin G-binding protein antibody
    • SPG2_STRSG antibody
    see all

References for Anti-Protein G antibody (HRP) (ab18599)

ab18599 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"