Purification notesAfter multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein G agarose and conjugated with horseradish peroxidase (HRP).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000.
1/5000 - 1/20000.
RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cellular localizationCell Wall and Secreted
IgG binding protein G antibody
IgG-binding protein G antibody
Immunoglobulin G binding protein G [Precursor] antibody
spg immunoglobulin G-binding protein antibody
References for Anti-Protein G antibody (HRP) (ab18599)
has not yet been referenced specifically in any publications.
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