Anti-Protein Kinase A regulatory subunit I alpha antibody (ab65013)

Overview

  • Product nameAnti-Protein Kinase A regulatory subunit I alpha antibody
    See all Protein Kinase A regulatory subunit I alpha primary antibodies
  • Description
    Rabbit polyclonal to Protein Kinase A regulatory subunit I alpha
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from N-terminal of human Protein Kinase A regulatory subunit I alpha.

  • Positive control
    • HepG2 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Involvement in diseaseDefects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
    Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
    Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
  • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACRDYS1 antibody
    • ADOHR antibody
    • cAMP dependent protein kinase regulatory subunit alpha 1 antibody
    • cAMP dependent protein kinase regulatory subunit RIalpha antibody
    • cAMP dependent protein kinase type I alpha regulatory chain antibody
    • cAMP dependent protein kinase type I alpha regulatory subunit antibody
    • cAMP-dependent protein kinase type I-alpha regulatory subunit antibody
    • CAR antibody
    • Carney complex type 1 antibody
    • CNC 1 antibody
    • CNC antibody
    • CNC1 antibody
    • DKFZp779L0468 antibody
    • KAP0_HUMAN antibody
    • MGC17251 antibody
    • PKA RIA antibody
    • PKR 1 antibody
    • PKR1 antibody
    • PPNAD 1 antibody
    • PPNAD1 antibody
    • PRKAR 1 antibody
    • PRKAR1 antibody
    • PRKAR1A antibody
    • PRKAR1A/RARA FUSION GENE antibody
    • Protein kinase A type 1a regulatory subunit antibody
    • Protein kinase cAMP dependent regulatory type I alpha antibody
    • PTC2 CHIMERIC ONCOGENE, INCLUDED antibody
    • Tissue specific extinguisher 1 antibody
    • Tissue-specific extinguisher 1 antibody
    • TSE 1 antibody
    • TSE1 antibody
    see all

Anti-Protein Kinase A regulatory subunit I alpha antibody images

  • All lanes : Anti-Protein Kinase A regulatory subunit I alpha antibody (ab65013) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ICC/IF image of ab65013 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65013, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Protein Kinase A regulatory subunit I alpha antibody (ab65013)

ab65013 has not yet been referenced specifically in any publications.

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