Recombinant
RabMAb

Anti-Protein Kinase A regulatory subunit I alpha (phospho S83) antibody [EPMAYR1] (ab154851)

Overview

  • Product name
    Anti-Protein Kinase A regulatory subunit I alpha (phospho S83) antibody [EPMAYR1]
    See all Protein Kinase A regulatory subunit I alpha primary antibodies
  • Description
    Rabbit monoclonal [EPMAYR1] to Protein Kinase A regulatory subunit I alpha (phospho S83)
  • Host species
    Rabbit
  • Specificity
    ab154851 only detects Protein Kinase A regulatory subunit I alpha phosphorylated at serine 83.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues surrounding phospho-serine 83 of Human Protein Kinase A regulatory subunit I alpha (UniProt: P10644).

  • Positive control
    • Rat heart lysate; Human kidney and prostate tissues.
  • General notes

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.

Target

  • Tissue specificity
    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Involvement in disease
    Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
    Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
    Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
  • Sequence similarities
    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACRDYS1 antibody
    • ADOHR antibody
    • cAMP dependent protein kinase regulatory subunit alpha 1 antibody
    • cAMP dependent protein kinase regulatory subunit RIalpha antibody
    • cAMP dependent protein kinase type I alpha regulatory chain antibody
    • cAMP dependent protein kinase type I alpha regulatory subunit antibody
    • cAMP-dependent protein kinase type I-alpha regulatory subunit antibody
    • CAR antibody
    • Carney complex type 1 antibody
    • CNC 1 antibody
    • CNC antibody
    • CNC1 antibody
    • DKFZp779L0468 antibody
    • KAP0_HUMAN antibody
    • MGC17251 antibody
    • PKA RIA antibody
    • PKR 1 antibody
    • PKR1 antibody
    • PPNAD 1 antibody
    • PPNAD1 antibody
    • PRKAR 1 antibody
    • PRKAR1 antibody
    • PRKAR1A antibody
    • PRKAR1A/RARA FUSION GENE antibody
    • Protein kinase A type 1a regulatory subunit antibody
    • Protein kinase cAMP dependent regulatory type I alpha antibody
    • PTC2 CHIMERIC ONCOGENE, INCLUDED antibody
    • Tissue specific extinguisher 1 antibody
    • Tissue-specific extinguisher 1 antibody
    • TSE 1 antibody
    • TSE1 antibody
    see all

Images

  • All lanes : Anti-Protein Kinase A regulatory subunit I alpha (phospho S83) antibody [EPMAYR1] (ab154851) at 1/1000 dilution

    Lane 1 : Rat heart lysate; untreated
    Lane 2 : Rat heart lysate; treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa



    Lower panel shows labeling with a non-phospho Protein Kinase A regulatory subunit I alpha antibody.
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Protein Kinase A regulatory subunit I alpha with ab154851 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Protein Kinase A regulatory subunit I alpha with ab154851 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154851 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154851 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154851 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154851 showing +ve staining.

References

ab154851 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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