• Product nameAnti-Protein Kinase D2 antibody
    See all Protein Kinase D2 primary antibodies
  • Description
    Rabbit polyclonal to Protein Kinase D2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from Human Protein Kinase D2 around the phosphorylation site of serine 876 (R-I-SP-V-L).

  • Positive control
    • NIH/3T3 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab59372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 97, 100 kDa (predicted molecular weight: 97 kDa).
ELISA 1/5000.
IHC-P Use a concentration of 1 µg/ml.


  • FunctionConverts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • Cellular localizationCytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

Anti-Protein Kinase D2 antibody images

  • All lanes : Anti-Protein Kinase D2 antibody (ab59372) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins)
    Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins) with immunizing phosphopeptide

    Predicted band size : 97 kDa
    Observed band size : 97,100 kDa (why is the actual band size different from the predicted?)
  • Ab59372 staining human normal tonsil. Staining is localised to cytoplasmic compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Protein Kinase D2 antibody (ab59372)

ab59372 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59372.
Please use the links above to contact us or submit feedback about this product.