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Rabbit polyclonal to Protein Kinase D2 (phospho S876)
Detects endogenous levels of PKD2 only when phosphorylated at serine 876
Synthetic phosphopeptide derived from human PKD2 around the phosphorylation site of serine 876 (R-I-S
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
The antibody against non-phosphopeptide was removed by chromatography
using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Phosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
Western blot - Protein Kinase D2 (phospho S876) antibody (ab59417)
All lanes : Anti-Protein Kinase D2 (phospho S876) antibody (ab59417) at 1/500 dilution Lane 1 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) Lane 2 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) with blocking phosphopeptide Predicted band size : 97 kDa Observed band size : 97 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
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