Overview

  • Product name
    Anti-Protein Phosphatase 1 beta antibody
    See all Protein Phosphatase 1 beta primary antibodies
  • Description
    Rabbit polyclonal to Protein Phosphatase 1 beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-274 of Human Protein Phosphatase 1 beta.

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysate. Mouse brain whole cell lysate. HeLa cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC3672 antibody
    • PP 1B antibody
    • PP-1B antibody
    • PP1B antibody
    • PP1B_HUMAN antibody
    • PP1beta antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • Protein phosphatase 1 beta antibody
    • Protein phosphatase 1 catalytic subunit beta isoform antibody
    • Protein phosphatase 1 delta antibody
    • Protein phosphatase 1, catalytic subunit, beta isozyme antibody
    • Protein phosphatase 1, catalytic subunit, delta isoform antibody
    • Serine threonine protein phosphatase PP1 beta catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

Images

  • All lanes : Anti-Protein Phosphatase 1 beta antibody (ab154600) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : Hep G2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    7.5% SDS PAGE
  • Anti-Protein Phosphatase 1 beta antibody (ab154600) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Protein Phosphatase 1 beta with ab154600 at 1/500 dilution. Lower panel is co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling Protein Phosphatase 1 beta with ab154600 at 1/100 dilution.

References

This product has been referenced in:
  • Gershoni-Emek N  et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). ICC/IF . Read more (PubMed: 26598648) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154600.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up