Recombinant Anti-PPP1CB antibody [EP1804Y] (ab53315)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1804Y] to PPP1CB
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPP1CB antibody [EP1804Y]
See all PPP1CB primary antibodies -
Description
Rabbit monoclonal [EP1804Y] to PPP1CB -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, Mouse brain, and Rat brain lysates. IHC-P: Human cervical carcinoma, Rat kidney tissue, Mouse kidney tissue, Mouse cardiac muscle and Rat colon tissue. IP: Human fetal brain lysate. ICC/IF: HeLa and MCF-7 cells. Flow Cyt (Intra): HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1804Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
For unpurified use at 1/10000 - 1/20000. |
IP |
1/100.
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IHC-P |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/250. |
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). For unpurified use at 1/10000 - 1/20000. |
IP
1/100. |
IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/250. |
ICC/IF
1/250. |
Target
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Function
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Cellular localization
Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 5500 Human
- Entrez Gene: 19046 Mouse
- Entrez Gene: 25594 Rat
- Omim: 600590 Human
- SwissProt: P62140 Human
- SwissProt: P62141 Mouse
- SwissProt: P62142 Rat
- Unigene: 702907 Human
see all -
Alternative names
- MGC3672 antibody
- PP 1B antibody
- PP-1B antibody
see all
Images
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All lanes : Anti-PPP1CB antibody [EP1804Y] (ab53315) at 1/50000 dilution (purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Protein phosphatase 1 beta with purified ab53315 at 1/40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue).
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ab53315 (purified) at 1/100 dilution (2ug) immunoprecipitating Protein phosphatase 1 beta in Human fetal brain lysate.
Lane 1 (input): Human fetal brain lysate 10ug
Lane 2 (+): ab53315 & Human fetal brain lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53315 in Human fetal brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Unpurified ab53315 staining PPP1CB antibody in HeLa (human cervix adenocarcinoma) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000 dilution.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling PPP1CB with purified ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Anti-PPP1CB antibody [EP1804Y] (ab53315) at 1/20000 dilution (unpurified) + Jurkat cells at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 37 kDa -
Immunocytochemistry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling PPP1CB with Purified ab53315 at 1:250 dilution (1.6 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemical analysis of paraffin-embedded sections of Human cervical carcinoma labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
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Immunohistochemical analysis of paraffin-embedded sections of mouse cardiac muscle labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
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Immunohistochemical analysis of paraffin-embedded sections of rat colon labelling PPP1CB with unpurified ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
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Flow Cytometry (Intracellular) analysis of HeLa cells labelling PPP1CB with unpurified ab53315 at 1/20 dilution. Goat anti rabbit IgG (H&L) (FITC) at 1/150 dilution was used as the secondary antibody. Isotype control = Rabbit monoclonal IgG. Fixative = 2% paraformaldehyde.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab53315 has been referenced in 29 publications.
- Smith TC et al. Novel γ-sarcoglycan interactors in murine muscle membranes. Skelet Muscle 12:2 (2022). PubMed: 35065666
- Winans S et al. A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun 13:1474 (2022). PubMed: 35304442
- Fitzpatrick CJ et al. Junin Virus Activates p38 MAPK and HSP27 Upon Entry. Front Cell Infect Microbiol 12:798978 (2022). PubMed: 35463647
- Bartosova L et al. Quercetin alleviates diastolic dysfunction and suppresses adverse pro-hypertrophic signaling in diabetic rats. Front Endocrinol (Lausanne) 13:1029750 (2022). PubMed: 36568083
- Yano T et al. A microtubule-LUZP1 association around tight junction promotes epithelial cell apical constriction. EMBO J 40:e104712 (2021). PubMed: 33346378