Anti-Protein phosphatase 1 inhibitor 3C antibody (ab103300)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab103300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • FunctionActs as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types.
  • Sequence similaritiesContains 1 CBM21 (carbohydrate binding type-21) domain.
  • DomainThe N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase, glycogen synthase and phosphorylase kinase.
  • Post-translational
    modifications
    Ubiquitinated by NHLRC1/malin in a EPM2A/laforin-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000020089 antibody
    • Phosphatase 1, regulatory inhibitor subunit 5 antibody
    • PP1 subunit R5 antibody
    • ppp1r3c antibody
    • PPP1R5 antibody
    • PPR3C_HUMAN antibody
    • Protein phosphatase 1 regulatory subunit 3C antibody
    • Protein phosphatase 1 regulatory subunit 5 antibody
    • protein phosphatase 1, regulatory (inhibitor) subunit 3C antibody
    • Protein targeting to glycogen antibody
    • PTG antibody
    • R5 antibody
    see all

Anti-Protein phosphatase 1 inhibitor 3C antibody images

  • Anti-Protein phosphatase 1 inhibitor 3C antibody (ab103300) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size : 37 kDa
  • All lanes : Anti-Protein phosphatase 1 inhibitor 3C antibody (ab103300) at 5 µg/ml

    Lane 1 : Protein phosphatase 1 inhibitor 3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa

References for Anti-Protein phosphatase 1 inhibitor 3C antibody (ab103300)

ab103300 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Tissue lysate - whole (myocardium)
Loading amount 50 µg
Specification myocardium
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Rahul Dalal

Verified customer

Submitted Sep 13 2012

Thank you again for your call last week and for your patience while I have reviewed your images and these proteins.

For ab18672 (IL8), I think the diffuse bands are caused bya bittoo much proteinloaded in the lanes orfrom running the gel at t...

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Thank you very much for sending these images. I am still reviewing the images and some information about the protein targets, but I will be back in touch shortly.

I am sorry for the delay, but please let me know if there is anything you need ...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please send any images of the blots and we will see if there are some suggestions to improve the results. Could you also...

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DISCOUNT CODE: ***
Expiration date: October, 15, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab103300using pig samplesand include this code in the &l...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"