Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)

Overview

  • Product name
    Anti-Protein phosphatase 1 inhibitor subunit 2 antibody
    See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies
  • Description
    Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2
  • Specificity
    ab73948 detects endogenous levels of total Protein phosphatase 1 inhibitor subunit 2 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Protein phosphatase 1 inhibitor subunit 2 around the phosphorylation site of serine 120 and serine 121 (Q-E-SP-SP-G-E).

  • Positive control
    • Human breast carcinoma tissue. Extracts from Jurkat cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Inhibitor of protein-phosphatase 1.
  • Sequence similarities
    Belongs to the protein phosphatase inhibitor 2 family.
  • Post-translational
    modifications
    Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • IPP 2 antibody
    • IPP-2 antibody
    • IPP2 antibody
    • IPP2_HUMAN antibody
    • MGC87148 antibody
    • Phosphatase inhibitor 2 antibody
    • Phosphoprotein phosphatase antibody
    • Ppp1r2 antibody
    • PPP1R2 protein antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 2 antibody
    • Protein phosphatase 1 regulatory subunit 2 antibody
    • Protein phosphatase inhibitor 2 antibody
    see all

Images

  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab73948, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References

ab73948 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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