Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850)

Overview

  • Product name
    Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
    See all Protein phosphatase 1 inhibitor subunit 2 primary antibodies
  • Description
    Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
  • Specificity
    ab27850 recognises Inhibitor-2.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide (Human) - derived from the region that contains threonine 72.

  • Positive control
    • HeLa or RAW 264.7 cells treated with nocodazole [200ng/ml] for 16 hours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, 1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions]. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
ICC 1/100.

Target

  • Function
    Inhibitor of protein-phosphatase 1.
  • Sequence similarities
    Belongs to the protein phosphatase inhibitor 2 family.
  • Post-translational
    modifications
    Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • IPP 2 antibody
    • IPP-2 antibody
    • IPP2 antibody
    • IPP2_HUMAN antibody
    • MGC87148 antibody
    • Phosphatase inhibitor 2 antibody
    • Phosphoprotein phosphatase antibody
    • Ppp1r2 antibody
    • PPP1R2 protein antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 2 antibody
    • Protein phosphatase 1 regulatory subunit 2 antibody
    • Protein phosphatase inhibitor 2 antibody
    see all

Images

  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution

    Lane 1 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with NO added peptide
    Lane 2 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with non-phosphopeptide corresponding to the immunogen
    Lane 3 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with generic phosphothreonine containing peptide
    Lane 4 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with phosphopeptide immunogen

    Secondary
    F(ab')2 ant-rabbit IgG HRP conjugate
    Developed using the ECL technique

    Predicted band size : 23 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution

    Lane 1 : HeLa cell extract with NO nocodazole
    Lane 2 : HeLa cell extract with 200ng/ml nocodazole for 16 hours
    Lane 3 : RAW 264.7 cell extract with NO nocodazole
    Lane 4 : RAW 264.7 cell extract with 200ng/ml nocodazole for 16 hours
    Lane 5 : HeLa cell extract with an antibody recognizing total human IPP2 protein
    Lane 6 : HeLa cell extract with an antibody recognizing total human IPP2 protein

    Secondary
    F(ab')2 ant-rabbit IgG HRP conjugate
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 44/46 kDa (possible dimer),46 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References

ab27850 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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