Anti-Protein phosphatase inhibitor 1 antibody [EP902Y] (ab40877)

Overview

  • Product nameAnti-Protein phosphatase inhibitor 1 antibody [EP902Y]
    See all Protein phosphatase inhibitor 1 primary antibodies
  • Description
    Rabbit monoclonal [EP902Y] to Protein phosphatase inhibitor 1
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Protein phosphatase inhibitor 1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • Brain tissue/lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab40877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Detects a band of approximately 27 kDa (predicted molecular weight: 19 kDa).
IP 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.
  • Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
  • Post-translational
    modifications
    Phosphorylation of Thr-35 is required for activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • I 1 antibody
    • I-1 antibody
    • I1 antibody
    • Inhibitor 1 antibody
    • IPP 1 antibody
    • IPP-1 antibody
    • IPP1 antibody
    • Ppp1r1a antibody
    • PPR1A_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1A antibody
    • Protein phosphatase 1 regulatory subunit 1A antibody
    • Protein phosphatase inhibitor 1 antibody
    see all

Anti-Protein phosphatase inhibitor 1 antibody [EP902Y] images

  • Anti-Protein phosphatase inhibitor 1 antibody [EP902Y] (ab40877) at 1/40000 dilution + Rat brain at 10 µg

    Predicted band size : 19 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human normal brain tissue using ab40877 diluted 1:100.
  • ICC/IF image of ab40877 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40877, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing SH-SY5Y cells stained with ab40877 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40877, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Protein phosphatase inhibitor 1 antibody [EP902Y] (ab40877)

This product has been referenced in:
  • Picard N  et al. Protein phosphatase 1 inhibitor-1 deficiency reduces phosphorylation of renal NaCl cotransporter and causes arterial hypotension. J Am Soc Nephrol 25:511-22 (2014). WB, IHC ; Mouse . Read more (PubMed: 24231659) »
  • Chao LC  et al. Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization. J Lipid Res 53:2610-9 (2012). WB ; Mouse . Read more (PubMed: 23028113) »

See all 4 Publications for this product

Product Wall

The peptide used is derived from the general region of 1-25 aa at the N-terminus.

Thank you for your enquiry. We have never directly tested this, but according to sequence alignment, ab40877 will not cross react with darpp32. If you have any other questions, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"