Abcam's Protein Quantitation Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in solution. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
|Protein Assay Solution||NM||1 x 20ml|
|Protein Standard||Yellow||1 x 1.5ml|
Our Abpromise guarantee covers the use of ab102535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.
Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted