Overview

  • Product nameAnti-Protein S antibody
    See all Protein S primary antibodies
  • Description
    Rabbit polyclonal to Protein S
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 209 and 447 of Human protein S (NP_000304).

  • Positive control
    • 293T and HeLa cell lysates for WB; HeLa cells for IF

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab97387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 75 kDa.
ICC/IF 1/100 - 1/200.
Flow Cyt Use at an assay dependent concentration. PubMed: 36558ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAnticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity.
  • Sequence similaritiesContains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.
  • Post-translational
    modifications
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Preproprotein S antibody
    • Propiece of latent protein S antibody
    • PROS 1 antibody
    • PROS antibody
    • PROS_HUMAN antibody
    • proS1 antibody
    • Protein S alpha antibody
    • Protein Sa antibody
    • PS 21 antibody
    • PS 22 antibody
    • PS 23 antibody
    • PS 24 antibody
    • PS 25 antibody
    • PS 26 antibody
    • PS21 antibody
    • PS22 antibody
    • PS23 antibody
    • PS24 antibody
    • PS25 antibody
    • PS26 antibody
    • PSA antibody
    • THPH5 antibody
    • THPH6 antibody
    • Vitamin K dependent protein S antibody
    • Vitamin K-dependent plasma protein S antibody
    • Vitamin K-dependent protein S antibody
    see all

Anti-Protein S antibody images

  • Anti-Protein S antibody (ab97387) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 75 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97387 at 1/200 dilution. Lower image merged with DNA probe.

References for Anti-Protein S antibody (ab97387)

ab97387 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophages)
Specification primary human macrophages
Treatment MDP 100ug/ml
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 18 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation BD #554722
Sample Human Cell (primary human monocyte)
Specification primary human monocyte
Gating Strategy macrophages
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jun 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"