Overview

  • Product name
    Anti-Protein Z antibody
  • Description
    Rabbit polyclonal to Protein Z
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Protein Z.

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Heart; Human Spleen; Human Bone Tumour; Human Bone Marrow. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab85317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).

Target

  • Function
    Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles.
  • Tissue specificity
    Plasma.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Z, vitamin K dependent plasma glycoprotein antibody
    • PROZ antibody
    • PROZ_HUMAN antibody
    • PZ antibody
    • Vitamin K-dependent protein Z antibody
    see all

Images

  • All lanes : Anti-Protein Z antibody (ab85317) at 1 µg/ml (Milk Blocking - 5%)

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human bone tumor tissue lysate - total protein (ab29359)
    Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa,55 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85317 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85317 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab85317 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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