Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab66068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.
  • Tissue specificity
    Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • coagulation factor II (thrombin) receptor-like 2 antibody
    • Coagulation factor II receptor-like 2 antibody
    • F2RL2 antibody
    • PAR 3 antibody
    • PAR-3 antibody
    • PAR3 antibody
    • PAR3_HUMAN antibody
    • protease activated receptor 3 antibody
    • Proteinase-activated receptor 3 antibody
    • thrombin receptor like 2 antibody
    • Thrombin receptor-like 2 antibody
    see all

Images

  • Anti-Proteinase Activated Receptor 3 antibody (ab66068) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 5 minutes

References

This product has been referenced in:
  • Wang T  et al. Cytokinesis defines a spatial landmark for hepatocyte polarization and apical lumen formation. J Cell Sci 127:2483-92 (2014). Mouse . Read more (PubMed: 24706948) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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