Anti-Proteus spp antibody [31-17] (ab8469)
Key features and details
- Mouse monoclonal [31-17] to Proteus spp
- Suitable for: ICC/IF
- Isotype: IgG2a
Overview
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Product name
Anti-Proteus spp antibody [31-17] -
Description
Mouse monoclonal [31-17] to Proteus spp -
Host species
Mouse -
Specificity
This antibody reacts with approximately 50% of the Proteus spp. isolates tested -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Proteus -
General notes
This antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
This product is sterile filtered. -
Primary antibody notes
This antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp. -
Clonality
Monoclonal -
Clone number
31-17 -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
Detection by luminescence, polymerase chain reaction or culture. Magnisort-M particles (DuPont) are diluted 25 times in PBS (+ 1% gelatin). 50 µl is transferred into a microtiterplate well. Add 50-75 µl antibody solution, incubate shaking 15 min. at room temperature. Magnetize plate and remove supernatant. Add 75 µl bacteria suspension and incubate shaking 15 min. at room temperature. Magnetize plate and wash beads three times with PBS-gelatin. Resuspend beads in appropriate volume and prepare for specific detection. |
Notes |
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ICC/IF
Use at an assay dependent concentration. Detection by luminescence, polymerase chain reaction or culture. Magnisort-M particles (DuPont) are diluted 25 times in PBS (+ 1% gelatin). 50 µl is transferred into a microtiterplate well. Add 50-75 µl antibody solution, incubate shaking 15 min. at room temperature. Magnetize plate and remove supernatant. Add 75 µl bacteria suspension and incubate shaking 15 min. at room temperature. Magnetize plate and wash beads three times with PBS-gelatin. Resuspend beads in appropriate volume and prepare for specific detection. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab8469 has not yet been referenced specifically in any publications.