Anti-Prothrombin antibody [BDI095] (ab22700)

Overview

  • Product name
    Anti-Prothrombin antibody [BDI095]
    See all Prothrombin primary antibodies
  • Description
    Mouse monoclonal [BDI095] to Prothrombin
  • Specificity
    ab22700 binds human prothrombin, specifically fragment 2.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human). Purified Human Prothrombin

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.15M Sodium chloride, 10mM Soudium dihydrogen phosphate, 1% Mannitol. pH 7.4
  • Concentration information loading...
  • Purification notes
    Antibody was purified by Gel filtration and ion exchange chromatography and the purity determined by 10% SDS-PAGE.
  • Clonality
    Monoclonal
  • Clone number
    BDI095
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 1 - 5 µg/ml. WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Involvement in disease
      Defects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
      Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
      Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
    • Sequence similarities
      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Form
      Cleaved into the following 4 chains: 1. Activation peptide fragment 1 2. Activation peptide fragment 2 3. Thrombin light chain 4. Thrombin heavy chain
    • Alternative names
      • coagulation factor II (thrombin) antibody
      • Coagulation factor II antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • prothrombin B-chain antibody
      • PT antibody
      • RPRGL2 antibody
      • serine protease antibody
      • THPH1 antibody
      • THRB antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all

    References for Anti-Prothrombin antibody [BDI095] (ab22700)

    ab22700 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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