Anti-Prothrombin antibody (Biotin) (ab49554)

Overview

  • Product nameAnti-Prothrombin antibody (Biotin)
    See all Prothrombin primary antibodies
  • Description
    Rabbit polyclonal to Prothrombin (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: RIA, IP, WB, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein purified from plasma (Human)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Competitive ELISA Use at an assay dependent dilution.

Target

  • FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
    Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • FormCleaved into the following 4 chains: 1. Activation peptide fragment 1 2. Activation peptide fragment 2 3. Thrombin light chain 4. Thrombin heavy chain
  • Alternative names
    • coagulation factor II (thrombin) antibody
    • Coagulation factor II antibody
    • F2 antibody
    • Factor II antibody
    • Prepro coagulation factor II antibody
    • Prothrombin antibody
    • prothrombin B-chain antibody
    • PT antibody
    • RPRGL2 antibody
    • serine protease antibody
    • THPH1 antibody
    • THRB antibody
    • THRB_HUMAN antibody
    • Thrombin heavy chain antibody
    see all

References for Anti-Prothrombin antibody (Biotin) (ab49554)

ab49554 has not yet been referenced specifically in any publications.

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We do have not tested antibodies which recognizes carboxylated form of Prothrombin.

ab49709 seems a closest match.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"