Anti-Prothrombin antibody [EPR5188] (ab109087)

Overview

  • Product nameAnti-Prothrombin antibody [EPR5188]
    See all Prothrombin primary antibodies
  • Description
    Rabbit monoclonal [EPR5188] to Prothrombin
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Prothrombin.

  • Positive control
    • Human serum, plasma, and platelet lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR5188
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • Tissue specificityExpressed by the liver and secreted in plasma.
    • Involvement in diseaseDefects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
      Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
      Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
    • Sequence similaritiesBelongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    • Cellular localizationSecreted > extracellular space.
    • Information by UniProt
    • Database links
    • FormCleaved into the following 4 chains: 1. Activation peptide fragment 1 2. Activation peptide fragment 2 3. Thrombin light chain 4. Thrombin heavy chain
    • Alternative names
      • coagulation factor II (thrombin) antibody
      • Coagulation factor II antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • prothrombin B-chain antibody
      • PT antibody
      • RPRGL2 antibody
      • serine protease antibody
      • THPH1 antibody
      • THRB antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all

    Anti-Prothrombin antibody [EPR5188] images

    • All lanes : Anti-Prothrombin antibody [EPR5188] (ab109087) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 70 kDa

    References for Anti-Prothrombin antibody [EPR5188] (ab109087)

    ab109087 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    We do have not tested antibodies which recognizes carboxylated form of Prothrombin.

    ab49709 seems a closest match.

    I hope this information is helpful to you. Please do not hesitate to contact...

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    Thank you for contacting us.

    I have checked all prothrombin antibodies in our catalog, but for none of them we know for sure that they will bind specifically to the carboxyglutamated form.

    As for ab109087, I found out that the immu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"