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Figure 1. Hemocytometer gridlines.
Hemocytometer diagram indicating the 16 corner squares which should be used for counting.
The trypan blue is used to stain any dead cells. Cells looking faint or dark blue within the grid being counted are counted as dead cells. To check the viability of the cells required:
Live cell count (not including trypan blue cells)
Total cell count including those stained with trypan blue
Live cell count = percentage viability
Total cell count
45 x 104/mL = percentage viability = 97.8% viability
46 x 104/mL