Overview

  • Product nameAnti-PROX1 antibody [5G10]
    See all PROX1 primary antibodies
  • Description
    Mouse monoclonal [5G10] to PROX1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment derived from within residues 500 - 600 fused to GST of Human PROX1.

  • Positive control
    • This antibody gave a positive result in HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate .

Properties

Applications

Our Abpromise guarantee covers the use of ab33219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTranscription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • Tissue specificityMost actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • DomainThe prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • Cellular localizationNucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • Database links
  • FormProx1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • Alternative names
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Anti-PROX1 antibody [5G10] images

  • All lanes : Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml

    Lane 1 : HepG2 nuclear extract lysate (ab14660)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 83 kDa


    Exposure time : 20 minutes
  • ab33219, at a 1/50 dilution, staining human PROX1 in pancreas tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

  • Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml + P3 Dentate gyrus whole tissue lysate at 50 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Image courtesy of an Abreview submitted by Dr. Xia Chen

    See Abreview

References for Anti-PROX1 antibody [5G10] (ab33219)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (gut)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 citrate, pH6.0
Permeabilization No
Specification gut
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 22 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

Submitted May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (P3 Dentate Gyrus)
Loading amount 50 µg
Specification P3 Dentate Gyrus
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Xia Chen

Verified customer

Submitted Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"