The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/750 - 1/1000.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
Use at an assay dependent concentration.
FunctionTranscription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
Tissue specificityMost actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the Prospero homeobox family. Contains 1 Prospero-type homeobox DNA-binding domain.
DomainThe prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
Cellular localizationNucleus. RORG promotes its nuclear localization.
FormProx1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.