Overview

  • Product name
  • Description
    Rabbit polyclonal to PROX1
  • Specificity
    We have had mixed results for use of this antibody in IHC-P. The abReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. Thus, we are removing IHC-P as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-P.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PROX1.

    (Peptide available as ab38691.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates : HepG2 and SHSY-5Y. HEK293 human whole cell lysate is a negative control lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).

Target

  • Function
    Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • Tissue specificity
    Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • Domain
    The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • Cellular localization
    Nucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • Database links
  • Form
    Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • Alternative names
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Images

  • All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)

    Lane 1 : HepG2 (Human) Whole Cell Lysate (ab52257)
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
    Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

This product has been referenced in:
  • Zhang Y  et al. Crim1 regulates integrin signaling in murine lens development. Development 143:356-66 (2016). IHC . Read more (PubMed: 26681494) »
  • Mori M  et al. Pulmonary and pleural lymphatic endothelial cells from pediatric, but not adult, patients with Gorham-Stout disease and generalized lymphatic anomaly, show a high proliferation rate. Orphanet J Rare Dis 11:67 (2016). IHC . Read more (PubMed: 27194137) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
testis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (gut)
Antigen retrieval step
Heat mediated
Specification
gut
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 29 2015



Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab38692 in Schweinegewebezu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einse...

Read More

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Leider sind Schwein und Maus oder Humanes Proteinfür Anti-Podoplanin / gp36 und CD31 nicht sehr homolog. Deshalb kann ich Ihnen auch keine Antikörper ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Jul 27 2012

Thank you for contacting us.

The anti-PROX1 antibody ab38692 is $347 for 100 µg+ $41 shipping and handling in the US. The antibody comes as a liquid, 100 µg at 1mg/ml, so you receive 100 µl.

http://www.abcam.com/p...

Read More

Merci pour votre émail. Nous avons plusieurs alternatives pour cet anticorps, mais ça dépends dans quel application et sur quel espèce le client veux détecter le PROX I. Le ab33219 est probablement le meilleur choix de remplacement, pourvu que le clien...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU)
Specification
E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Dr. Stephanie Cossette

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (P3 Dentate Gyrus)
Loading amount
50 µg
Specification
P3 Dentate Gyrus
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Xia Chen

Verified customer

Submitted Apr 01 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 08 2010

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up