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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PROX1.
(Peptide available as ab38691.)
Our Abpromise guarantee covers the use of ab38692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||Use at an assay dependent concentration.|
Formaldehyde-fixed, paraffin-embedded human heart tissue stained for PROX1 using ab388692 at 1/250 dilution in immunohistochemical analysis. Incubation with primary antibody was performed for 45 minutes at 20°C. Goat anti-rabbit/mouse HRO polymer was used as a secondary antibody.
Heat mediated antigen retrieval was performed. Tissue sections were blocked with peroxidase for 5 minutes followed by a 10 minute protein block.