Anti-PROX1 antibody [EPR3128(2)] (ab124910)

Overview

  • Product nameAnti-PROX1 antibody [EPR3128(2)]
    See all PROX1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3128(2)] to PROX1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PROX1.

  • Positive control
    • HepG2 and SHSY-5Y cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionTranscription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
    • Tissue specificityMost actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
    • Sequence similaritiesBelongs to the Prospero homeobox family.
      Contains 1 Prospero-type homeobox DNA-binding domain.
    • DomainThe prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
    • Cellular localizationNucleus. RORG promotes its nuclear localization.
    • Information by UniProt
    • Database links
    • FormProx1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
    • Alternative names
      • Homeobox prospero like protein antibody
      • Homeobox prospero like protein PROX1 antibody
      • Homeobox prospero-like protein PROX1 antibody
      • Prospero homeobox 1 antibody
      • Prospero homeobox protein 1 antibody
      • Prospero related homeobox 1 antibody
      • prospero-related homeobox gene 1 antibody
      • PROX 1 antibody
      • PROX-1 antibody
      • PROX1 antibody
      • PROX1_HUMAN antibody
      • zgc:111888 antibody
      see all

    Anti-PROX1 antibody [EPR3128(2)] images

    • All lanes : Anti-PROX1 antibody [EPR3128(2)] (ab124910) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SHSY-5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 83 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-PROX1 antibody [EPR3128(2)] (ab124910)

    ab124910 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (gut)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization No
    Specification gut
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

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    Verified customer

    Submitted May 26 2015

    Je tiens à vous informer que le laboratoire n'a pas non plus pu retrouver la réactivité contre la souris et nous allons donc enlever ceci de notre fiche technique. Nous allons aussi informer les clients concernés.

    ...

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    Merci beaucoup pour votreémail. Je suis désolée d'entendre que le nouveau tube n'as pas donné des bons résultats. Je vais mettre en place la procédure pour le remboursement et vous envoyer un émail avec les détails sous peu.

    Pouvez vous s'il ...

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    Merci pour votre réponse.

    C'est possible que le problème est une question de sensibilité. Est-ce que vous pourriezpeut-être charger plus de lysat sur le gel? J'espère que la BSA va aider aussi. Je comprends a...

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    Merci beaucoup de nous avoir envoyé ces documents. Je suis désolée que vous avez eu des problèmes avec cet anticorps.

    J'ai contacté les différents laboratoires. Je peux confirmer que nous avions retes...

    Read More

    Je suis désolée d'apprendre que le ab124910 ne vous donne pas de bons résultats en Western blot et ne compare pas les résultats du ab11941. Je suis désolée que nous ne pouvons plus avoir du ab11941.

    C...

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