Overview

  • Product name
  • Description
    Rabbit polyclonal to PRP19
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human PRP19.

    (Peptide available as ab28710.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab27692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP Use at an assay dependent concentration. PubMed: 20599946
ICC/IF 1/500.

Target

  • Function
    Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
  • Tissue specificity
    Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
  • Sequence similarities
    Belongs to the WD repeat PRP19 family.
    Contains 1 U-box domain.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPso4 antibody
    • NMP200 antibody
    • Nuclear matrix protein 200 antibody
    • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
    • pre mRNA processing factor 19 antibody
    • Pre-mRNA-processing factor 19 antibody
    • PRP19 antibody
    • PRP19/PSO4 homolog antibody
    • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
    • PRP19_HUMAN antibody
    • PRPF19 antibody
    • PSO4 antibody
    • psoralen 4 antibody
    • Senescence evasion factor antibody
    • SNEV antibody
    • UBOX4 antibody
    see all

Anti-PRP19 antibody images

  • All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Ab27692, at 1/500 dilution, staining human PRP 19 in HeLa Cells by ICC/IF.

    See Abreview

  • All lanes : Anti-PRP19 antibody (ab27692) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Image courtesy of Human Protein Atlas. ab27692 staining PRP19 in human breast cancer, showing a distinct nuclear staining pattern. Paraffin embedded human breast cancer tissue was incubated with ab27692 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab27692 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

References for Anti-PRP19 antibody (ab27692)

This product has been referenced in:
  • van Maldegem F  et al. CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. Nucleic Acids Res 43:7058-69 (2015). WB, ICC/IF ; Mouse . Read more (PubMed: 26130721) »
  • Maréchal A  et al. PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry. Mol Cell 53:235-46 (2014). Human . Read more (PubMed: 24332808) »

See all 6 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - other (Hek 293T)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
1e+007 cells
Treatment
UV 50j/m2
Specification
Hek 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS ATCC)
Permeabilization
Yes - 0.5% Triton X-100 in PBS 1X
Specification
U2OS ATCC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
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Submitted Oct 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (egg extract with sperm nuclei)
Specification
egg extract with sperm nuclei
Fixative
Formaldehyde
Permeabilization
Yes - 100 % methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

Submitted Oct 19 2011

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (egg extract)
Loading amount
50 µg
Specification
egg extract
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Dec 18 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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