Overview

  • Product nameAnti-PRP19 antibody
    See all PRP19 primary antibodies
  • Description
    Mouse monoclonal to PRP19
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF , corresponding to amino acids 1-91 of Human PRP19

  • Positive control
    • Hela, PC12 and NIH3T3 cells (rat and mouse cells have a lower band <30kDa in addition).

Properties

Applications

Our Abpromise guarantee covers the use of ab57275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
    • Tissue specificityUbiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
    • Sequence similaritiesBelongs to the WD repeat PRP19 family.
      Contains 1 U-box domain.
      Contains 7 WD repeats.
    • Cellular localizationNucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • hPso4 antibody
      • NMP200 antibody
      • Nuclear matrix protein 200 antibody
      • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
      • pre mRNA processing factor 19 antibody
      • Pre-mRNA-processing factor 19 antibody
      • PRP19 antibody
      • PRP19/PSO4 homolog antibody
      • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
      • PRP19_HUMAN antibody
      • PRPF19 antibody
      • PSO4 antibody
      • psoralen 4 antibody
      • Senescence evasion factor antibody
      • SNEV antibody
      • UBOX4 antibody
      see all

    Anti-PRP19 antibody images



    • Predicted band size : 55 kDa
      PRP19 antibody (ab57275) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-PRP19 antibody (ab57275)

    ab57275 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Schizosaccharomyces pombe Recombinant protein (purified His tagged S.pombe PRP19 from E.coli)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 2 µg
    Specification purified His tagged S.pombe PRP19 from E.coli
    Blocking step odyssey blocking buffer,927-40000 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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    Verified customer

    Submitted Apr 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"