Overview

  • Product name
    Anti-PRP19 antibody [EPR7446]
    See all PRP19 primary antibodies
  • Description
    Rabbit monoclonal [EPR7446] to PRP19
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PRP19.

  • Positive control
    • Human breast carcinoma and colon carcinoma tissues; HepG2, A431, MCF7, PC12, NIH 3T3, HeLa, K562, and U937 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
    • Tissue specificity
      Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.
    • Sequence similarities
      Belongs to the WD repeat PRP19 family.
      Contains 1 U-box domain.
      Contains 7 WD repeats.
    • Cellular localization
      Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin.
    • Information by UniProt
    • Database links
    • Alternative names
      • hPso4 antibody
      • NMP200 antibody
      • Nuclear matrix protein 200 antibody
      • Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
      • pre mRNA processing factor 19 antibody
      • Pre-mRNA-processing factor 19 antibody
      • PRP19 antibody
      • PRP19/PSO4 homolog antibody
      • PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
      • PRP19_HUMAN antibody
      • PRPF19 antibody
      • PSO4 antibody
      • psoralen 4 antibody
      • Senescence evasion factor antibody
      • SNEV antibody
      • UBOX4 antibody
      see all

    Images

    • All lanes : Anti-PRP19 antibody [EPR7446] (ab126776) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : HeLa cell lysate
      Lane 7 : K562 cell lysate
      Lane 8 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 55 kDa
    • ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab126776, at 1/50 dilution, staining PRP19 in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Guo J  et al. Unraveling molecular effects of ADAR1 overexpression in HEK293T cells by label-free quantitative proteomics. Cell Cycle 15:1591-601 (2016). Read more (PubMed: 27104882) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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