1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInvolved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6 with ab99292 at 1/200 (1µg/ml). Detection: DAB.
Western blot - PRP6 antibody (ab99292)
All lanes : Anti-PRP6 antibody (ab99292) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 107 kDa
Exposure time : 30 seconds
Immunoprecipitation - PRP6 antibody (ab99292)
Lane 1 : Detection of Human PRP6 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3µg/ml ab99292.
Lane 2: Control IgG
References for Anti-PRP6 antibody (ab99292)
This product has been referenced in:
Liu YC et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog10:e1004199 (2014).
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