Overview

  • Product name
  • Description
    Rabbit polyclonal to PRP6
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 1 and 50 of Human PRP6 (NP_036601.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab99292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.
  • Sequence similarities
    Contains 9 HAT repeats.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen receptor N terminal domain transactivating protein 1 antibody
    • ANT 1 antibody
    • ANT1 antibody
    • C20orf14 antibody
    • hPrp6 antibody
    • p102 U5 small nuclear ribonucleoprotein particle binding protein antibody
    • pre-mRNA processing factor 6 antibody
    • Pre-mRNA-processing factor 6 antibody
    • PRP6 homolog antibody
    • PRP6 pre mRNA processing factor 6 homolog antibody
    • PRP6_HUMAN antibody
    • PRPF6 antibody
    • Putative mitochondrial outer membrane protein import receptor antibody
    • TOM antibody
    • U5 snRNP-associated 102 kDa protein antibody
    • U5-102 kDa protein antibody
    • U5-102K antibody
    see all

Anti-PRP6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6 with ab99292 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PRP6 antibody (ab99292) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 107 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human PRP6 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3µg/ml ab99292. Lane 2: Control IgG

References for Anti-PRP6 antibody (ab99292)

This product has been referenced in:
  • Cloutier P  et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . Read more (PubMed: 28561026) »
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab99292.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up