Overview

  • Product nameAnti-PRPF3 antibody
    See all PRPF3 primary antibodies
  • Description
    Rabbit polyclonal to PRPF3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: VDKLFEAVEE GRSSRHSKSS SDRSRKRELK EVFGDDSEIS KESSGVKKRR, corresponding to amino acids 63-112 of Human PRPF3

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

Titre using peptide based assay 1/62500.

Target

Anti-PRPF3 antibody images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-PRPF3 antibody (ab50386) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 77 kDa
    Observed band size : 77 kDa

References for Anti-PRPF3 antibody (ab50386)

ab50386 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Hek 293T)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 1e+007 cells
Specification Hek 293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Nov 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS ATCC)
Permeabilization Yes - 0.5% Triton X-100 in PBS 1X
Specification U2OS ATCC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative Paraformaldehyde
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Submitted Oct 07 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"