Overview

  • Product nameAnti-PRPF31 antibody
    See all PRPF31 primary antibodies
  • Description
    Goat polyclonal to PRPF31
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    KELGNSLDKCKNNEN

    , corresponding to internal sequence amino acids 140-154 of Human PRPF31

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: antibody detection limit dilution 1:128,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

    An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.
    • Tissue specificityUbiquitously expressed.
    • Involvement in diseaseDefects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.
    • Sequence similaritiesBelongs to the PRP31 family.
      Contains 1 Nop domain.
    • DomainInteracts with the snRNP via the Nop domain.
      The coiled coil domain is formed by two non-contiguous helices.
    • Cellular localizationNucleus speckle. Nucleus > Cajal body. Predominantly found in speckles and in Cajal bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp566J153 antibody
      • hPrp 31 antibody
      • hPrp31 antibody
      • NY BR 99 antibody
      • Pre mRNA processing factor 31 antibody
      • Pre mRNA processing factor 31 homolog (yeast) antibody
      • Pre mRNA processing factor 31 homolog antibody
      • Pre-mRNA-processing factor 31 antibody
      • Precursor mRNA-processing factor 31, S. cerevisiae, homolog of antibody
      • Protein 61K antibody
      • PRP 31 antibody
      • PRP31 antibody
      • PRP31 pre mRNA processing factor 31 homolog (yeast) antibody
      • PRP31 pre mRNA processing factor 31 homolog antibody
      • PRP31_HUMAN antibody
      • PRPF 31 antibody
      • prpf31 antibody
      • RP 11 antibody
      • RP11 antibody
      • Serologically defined breast cancer antigen NY BR 99 antibody
      • Serologically defined breast cancer antigen NY-BR-99 antibody
      • SNRNP61 antibody
      • U4/U6 small nuclear ribonucleoprotein Prp31 antibody
      • U4/U6 snRNP 61 kDa protein antibody
      see all

    Anti-PRPF31 antibody images

    • All lanes : Anti-PRPF31 antibody (ab58588) at 1 µg/ml

      Lane 1 : HeLa cell lysate (35µg protein in RIPA buffer) without blocking with the immunizing peptide
      Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


      Predicted band size : 55 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-PRPF31 antibody (ab58588)

    ab58588 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"