Recombinant
RabMAb

Anti-PRPF31 antibody [EPR14587] (ab188577)

Overview

  • Product name
    Anti-PRPF31 antibody [EPR14587]
    See all PRPF31 primary antibodies
  • Description
    Rabbit monoclonal [EPR14587] to PRPF31
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRPF31 aa 350-450. The exact sequence is proprietary.
    Database link: Q8WWY3

  • Positive control
    • HeLa and Jurkat cell lysates; rat spleen lysate. HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Flow Cyt 1/240. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250.

Target

  • Function
    Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the PRP31 family.
    Contains 1 Nop domain.
  • Domain
    Interacts with the snRNP via the Nop domain.
    The coiled coil domain is formed by two non-contiguous helices.
  • Cellular localization
    Nucleus speckle. Nucleus > Cajal body. Predominantly found in speckles and in Cajal bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp566J153 antibody
    • hPrp 31 antibody
    • hPrp31 antibody
    • NY BR 99 antibody
    • Pre mRNA processing factor 31 antibody
    • Pre mRNA processing factor 31 homolog (yeast) antibody
    • Pre mRNA processing factor 31 homolog antibody
    • Pre-mRNA-processing factor 31 antibody
    • Precursor mRNA-processing factor 31, S. cerevisiae, homolog of antibody
    • Protein 61K antibody
    • PRP 31 antibody
    • PRP31 antibody
    • PRP31 pre mRNA processing factor 31 homolog (yeast) antibody
    • PRP31 pre mRNA processing factor 31 homolog antibody
    • PRP31_HUMAN antibody
    • PRPF 31 antibody
    • prpf31 antibody
    • RP 11 antibody
    • RP11 antibody
    • Serologically defined breast cancer antigen NY BR 99 antibody
    • Serologically defined breast cancer antigen NY-BR-99 antibody
    • SNRNP61 antibody
    • U4/U6 small nuclear ribonucleoprotein Prp31 antibody
    • U4/U6 snRNP 61 kDa protein antibody
    see all

Images

  • All lanes : Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Anti-PRPF31 antibody [EPR14587] (ab188577) at 1/1000 dilution + rat spleen at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PRPF31 with ab188577 at a 1/250 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

  • Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling PRPF31 with ab188577 at a 1/240 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

References

ab188577 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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