Overview

  • Product name
  • Description
    Mouse polyclonal to PRPF4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PRPF4 protein (Human), NP_004688.

  • Positive control
    • PRPF4 transfected 293T cell lysate and human spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
  • Sequence similarities
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus speckle. Colocalizes with spliceosomal snRNPs.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPrp4 antibody
    • HPRP4P antibody
    • PRP4 antibody
    • PRP4 homolog antibody
    • PRP4 pre mRNA processing factor 4 homolog (yeast) antibody
    • PRP4/STK/WD splicing factor antibody
    • PRP4_HUMAN antibody
    • Prp4p antibody
    • PRPF4 antibody
    • RP70 antibody
    • SNRNP60 antibody
    • U4/U6 small nuclear ribonucleoprotein Prp4 antibody
    • U4/U6 snRNP 60 kDa protein antibody
    • WD splicing factor Prp4 antibody
    see all

Images

  • Anti-PRPF4 antibody (ab69878) at 1/500 dilution + human spleen tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • All lanes : Anti-PRPF4 antibody (ab69878) at 1/500 dilution

    Lane 1 : PRPF4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References

ab69878 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon, breast)
Loading amount
5 µg
Specification
colon, breast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Soonil Koun

Verified customer

Submitted Mar 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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