Recombinant Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17207] to PRPF4 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PRPF4 antibody [EPR17207] - C-terminal
See all PRPF4 primary antibodies -
Description
Rabbit monoclonal [EPR17207] to PRPF4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, HepG2, 293, C6, Raw264.7, NIH/3T3 and HeLa whole cell lysate, Mouse brain kidney and spleen tissue lysates. IHC: Human transitional cell carcinoma of bladder tissue, Human kidney tissue, Rat cerebral cortex tissue, and Mouse cardiac muscle tissue. ICC/I: MCF-7 and HeLa cells. Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17207 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/70.
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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Notes |
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Flow Cyt (Intra)
1/70. |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. -
Sequence similarities
Contains 7 WD repeats. -
Cellular localization
Nucleus speckle. Colocalizes with spliceosomal snRNPs. - Information by UniProt
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Database links
- Entrez Gene: 9128 Human
- Entrez Gene: 70052 Mouse
- Entrez Gene: 298095 Rat
- Omim: 607795 Human
- SwissProt: O43172 Human
- SwissProt: Q9DAW6 Mouse
- Unigene: 744014 Human
- Unigene: 30660 Mouse
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Alternative names
- hPrp4 antibody
- HPRP4P antibody
- PRP4 antibody
see all
Images
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All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/20000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human transitional cell carcinoma of bladder tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human kidney tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophages transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 15 secondsBlocking and diluting buffer was 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Rat cerebral cortex tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Mouse cardiac muscle tissuewas observed (Subcellular location - Nucleus speckle [UniProt]).
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HeLa cells labelling PRPF4 (red) with purified ab198998 at dilution of 1/70. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab198998 has not yet been referenced specifically in any publications.