Recombinant
RabMAb

Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998)

Overview

  • Product name
    Anti-PRPF4 antibody [EPR17207] - C-terminal
    See all PRPF4 primary antibodies
  • Description
    Rabbit monoclonal [EPR17207] to PRPF4 - C-terminal
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PRPF4 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O43172

  • Positive control
    • WB: Raji, HepG2, 293, C6, Raw264.7, NIH/3T3 and HeLa whole cell lysate, Mouse brain kidney and spleen tissue lysates. IHC: Human transitional cell carcinoma of bladder tissue, Human kidney tissue, Rat cerebral cortex tissue, and Mouse cardiac muscle tissue. ICC/I: MCF-7 and HeLa cells. FC: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab198998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/70.
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF 1/500.

Target

  • Function
    Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
  • Sequence similarities
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus speckle. Colocalizes with spliceosomal snRNPs.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPrp4 antibody
    • HPRP4P antibody
    • PRP4 antibody
    • PRP4 homolog antibody
    • PRP4 pre mRNA processing factor 4 homolog (yeast) antibody
    • PRP4/STK/WD splicing factor antibody
    • PRP4_HUMAN antibody
    • Prp4p antibody
    • PRPF4 antibody
    • RP70 antibody
    • SNRNP60 antibody
    • U4/U6 small nuclear ribonucleoprotein Prp4 antibody
    • U4/U6 snRNP 60 kDa protein antibody
    • WD splicing factor Prp4 antibody
    see all

Images

  • All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/20000 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa


    Exposure time : 3 minutes

    Blocking and diluting buffer was 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PRPF4 with ab198998 at 1/500 followed by  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human transitional cell carcinoma of bladder tissue was observed (Subcellular location - Nucleus speckle [UniProt]).

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa


    Exposure time : 3 minutes

    Blocking and diluting buffer was 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Human kidney tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-PRPF4 antibody [EPR17207] - C-terminal (ab198998) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophages transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa


    Exposure time : 15 seconds

    Blocking and diluting buffer was 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Rat cerebral cortex tissue was observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PRPF4 with ab198998 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Counter stained with Hematoxylin. Nuclear staining on Mouse cardiac muscle tissuewas observed (Subcellular location - Nucleus speckle [UniProt]).
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Flow cytometry analysis of HeLa cells labelling PRPF4 (red) with purified ab198998 at dilution of 1/70. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab198998 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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