Overview

  • Product name
    Anti-PRPK antibody
  • Description
    Rabbit polyclonal to PRPK
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH, selected from the sequence of Human PRPK within amino acids 200-253.

  • Positive control
    • Mouse thymus tissue lysate, Human breast carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
  • Tissue specificity
    Highly expressed in testis. Weakly expressed in heart kidney and spleen.
  • Sequence similarities
    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf64 antibody
    • Nori 2 antibody
    • Nori 2p antibody
    • Nori-2 antibody
    • p53 related protein kinase antibody
    • p53-related protein kinase antibody
    • PRPK_HUMAN antibody
    • TP53 regulating kinase antibody
    • TP53-regulating kinase antibody
    • TP53RK antibody
    see all

Images

  • Anti-PRPK antibody (ab37606) at 1/100 dilution + Mouse thymus tissue lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 15-20 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ab37606, at 1/50 dilution, staining PRPK in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37606 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

This product has been referenced in:
  • Villarroel-Campos D  et al. Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. J Neurosci 36:7298-313 (2016). Read more (PubMed: 27383602) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab37606.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up