Anti-PRPS1 antibody (ab154721)
Key features and details
- Rabbit polyclonal to PRPS1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PRPS1 antibody
See all PRPS1 primary antibodies -
Description
Rabbit polyclonal to PRPS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-318 of Human PRPS1 (P60891)
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Positive control
- Recombinant Human PRPS1 protein (ab92935) can be used as a positive control in WB. 293T, Rat liver or Mouse kidney lysate, TOV-21G xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
Target
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Function
Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. -
Pathway
Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. -
Involvement in disease
Defects in PRPS1 are the cause of phosphoribosylpyrophosphate synthetase superactivity (PRPS1 superactivity) [MIM:300661]; also known as PRPS-related gout. It is a familial disorder characterized by excessive purine production, gout and uric acid urolithiasis.
Defects in PRPS1 are the cause of Charcot-Marie-Tooth disease X-linked recessive type 5 (CMTX5) [MIM:311070]; also known as optic atrophy-polyneuropathy-deafness or Rosenberg-Chutorian syndrome. CMTX5 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy.
Defects in PRPS1 are the cause of ARTS syndrome (ARTS) [MIM:301835]; also known as fatal ataxia X-linked with deafness and loss of vision. ARTS is a disorder characterized by mental retardation, early-onset hypotonia, ataxia, delayed motor development, hearing impairment, and optic atrophy. Susceptibility to infections, especially of the upper respiratory tract, can result in early death.
Defects in PRPS1 are the cause of deafness X-linked type 1 (DFNX1) [MIM:304500]; also known as congenital sensorineural deafness X-linked 2 (DFN2). It is a form of deafness characterized by progressive, severe-to-profound sensorineural hearing loss in males. Females manifest mild to moderate hearing loss. -
Sequence similarities
Belongs to the ribose-phosphate pyrophosphokinase family. - Information by UniProt
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Database links
- Entrez Gene: 5631 Human
- Entrez Gene: 19139 Mouse
- Entrez Gene: 29562 Rat
- Entrez Gene: 314140 Rat
- Omim: 311850 Human
- SwissProt: P60891 Human
- SwissProt: Q9D7G0 Mouse
- SwissProt: P60892 Rat
see all -
Alternative names
- ARTS antibody
- CMTX5 antibody
- Deafness 2 perceptive congenital antibody
see all
Images
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Anti-PRPS1 antibody (ab154721) at 1/1500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 34 kDa
7.5% SDS PAGE -
Anti-PRPS1 antibody (ab154721) at 1/10000 dilution + Rat liver whole cell lysate at 50 µg
Predicted band size: 34 kDa
10% SDS PAGE -
Anti-PRPS1 antibody (ab154721) at 1/3000 dilution + Mouse kidney whole cell lysate at 50 µg
Predicted band size: 34 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human TOV-21G xenograft tissue labeling PRPS1 with ab154721 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154721 has been referenced in 1 publication.
- Wang X et al. Purine synthesis promotes maintenance of brain tumor initiating cells in glioma. Nat Neurosci 20:661-673 (2017). PubMed: 28346452