Overview

  • Product name
  • Description
    Rabbit polyclonal to PRRT3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PLENSEIPMI PGAHPKGSVG SEPQAFDVFP ENPRADSHRN SDVRHAPAEE MPEKPVASPL GPALYGPKAA QGAQRERLPV TDDLQMAQGP SSH, corresponding to amino acids 33-125 of Human PRRT3 (UniProt Q5FWE3).

  • Positive control
    • WB: RT-4 cell and Human plasma lysates IHC-P: Human duodenum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane. Recommended concentration of ab122252 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122252, at a 1/250 dilution, staining PRRT3 in paraffin embedded Human duodenum tissue by Immunohistochemistry.

References

ab122252 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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