The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay play a role in the scarless healing of cutaneous wounds during the first two trimesters of development.
Tissue specificityIn fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis.
Sequence similaritiesBelongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain.
Developmental stageHigher expression in fetus than in adult.