Overview

  • Product nameAnti-PRRX2 antibody
    See all PRRX2 primary antibodies
  • Description
    Mouse monoclonal to PRRX2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 151-253 of Human PRRX2 with tag (NP_057391).

  • Positive control
    • PRRX2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionMay play a role in the scarless healing of cutaneous wounds during the first two trimesters of development.
  • Tissue specificityIn fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis.
  • Sequence similaritiesBelongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageHigher expression in fetus than in adult.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Paired mesoderm homeobox protein 2 antibody
    • Paired related homeobox protein 2 antibody
    • paired-like homeobox gene 2 antibody
    • paired-like homeodomain protein PRX2 antibody
    • Paired-related homeobox protein 2 antibody
    • PMX2 antibody
    • Predicted NUP98/PRRX2 fusion protein antibody
    • Prrx2 antibody
    • PRRX2_HUMAN antibody
    • PRX 2 antibody
    • PRX-2 antibody
    • PRX2 antibody
    see all

Anti-PRRX2 antibody images

  • Anti-PRRX2 antibody (ab77655) at 5 µg/ml + Recombinant fragment of human PRRX2 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PRRX2 antibody (ab77655) at 5 µg/ml

    Lane 1 : PRRX2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

References for Anti-PRRX2 antibody (ab77655)

ab77655 has not yet been referenced specifically in any publications.

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