Overview

  • Product name
  • Description
    Mouse polyclonal to PRSS2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PRSS2

  • Positive control
    • Human pancreas tissue lysate PRSS2 transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Sulfated on tyrosine.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anionic trypsinogen antibody
    • EC 3.4.21.4 antibody
    • MGC111183 antibody
    • MGC120174 antibody
    • Protease serine 2 preproprotein antibody
    • Protease, serine, 2 (trypsin 2) antibody
    • Protease, serine, 2 antibody
    • Protease, serine, 2, preproprotein antibody
    • Prss2 antibody
    • Serine protease 2 antibody
    • TRY2 antibody
    • TRY2_HUMAN antibody
    • TRY8 antibody
    • TRYP2 antibody
    • Trypsin 2 antibody
    • Trypsin II antibody
    • Trypsin-2 antibody
    • Trypsinogen 2 antibody
    see all

Anti-PRSS2 antibody images

  • ab76781 staining PRSS2 in Human primary motor cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 2 hours at 20°C; antigen retrieval was by heat mediation in tris EDTA, pH9.2. Samples were incubated with primary antibody (1/200 in 2% serum, 0.5% Triton X-100 in PBS) for 20 hours at 20°C. An undiluted Biotin-conjugated Sheep anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-PRSS2 antibody (ab76781) at 1/500 dilution + Human pancreas tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • All lanes : Anti-PRSS2 antibody (ab76781) at 1/500 dilution

    Lane 1 : PRSS2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PRSS2 antibody (ab76781)

ab76781 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris EDTA pH9.2
Sample
Human Tissue sections (primary motor cortex)
Specification
primary motor cortex
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Michael Hurley

Verified customer

Submitted Aug 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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