Overview

  • Product name
  • Description
    Rabbit polyclonal to PRSS3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PRSS3 aa 143-171 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P35030

  • Positive control
    • HL60 and MDA-MB231 cell lysates, Human pancreas tissue, HL60 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170361 is purified through a protein A column, followed by peptide affinity purification
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.

Target

  • Function
    Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
  • Tissue specificity
    Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain trypsinogen antibody
    • Mesotrypsin antibody
    • Mesotrypsinogen antibody
    • MTG antibody
    • Pancreatic trypsinogen III antibody
    • Protease, serine, 3 antibody
    • Protease, serine, 4 (trypsin 4, brain) antibody
    • PRSS3 antibody
    • PRSS4 antibody
    • Serine protease 3 antibody
    • Serine protease 4 antibody
    • T9 antibody
    • TRY3 antibody
    • TRY3_HUMAN antibody
    • TRY4 antibody
    • Trypsin 3 antibody
    • Trypsin III antibody
    • Trypsin IV antibody
    • Trypsin-3 antibody
    • Trypsinogen 4 antibody
    • Trypsinogen 5 antibody
    • Trypsinogen IV antibody
    see all

Images

  • Flow cytometry analysis of HL60 cells labeling PRSS3 (green, right histogram), using ab170361 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human pancreas tissue, labeling PRSS3 using ab170361 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-PRSS3 antibody (ab170361) at 1/100 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : MDA-MB231 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 33 kDa

References

This product has been referenced in:
  • Qian L  et al. PRSS3 is a prognostic marker in invasive ductal carcinoma of the breast. Oncotarget 8:21444-21453 (2017). IHC ; Human . Read more (PubMed: 28423522) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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