Overview

  • Product nameAnti-PRSS35 antibody
    See all PRSS35 primary antibodies
  • Description
    Rabbit polyclonal to PRSS35
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (TQNITTKGVS VRRKRQVYGT DSRFSILDKR FLTNFPFSTA VKLSTGCSGI) of Human PRSS35 (NP_699193).

  • Positive control
    • 721_B cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePRSS35, although related to the peptidase S1 family, lacks the conserved active Ser residue in position 346 which is replaced by a Thr, suggesting that it has no protease activity. This Ser to Thr substitution may play an important role in determining a unique pattern of substrate specificity for PRSS35. Alternatively, this substitution may yield an inactive protease that binds to specific substrates, thus protecting them from proteolysis. The Mouse Prss35 gene is selectively expressed in the ovary and it's expression prior to ovulation is, in part, regulated by progesterone. PRSS35 may be involved in ovulation and the formation and regression of the corpus luteum.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • 6030424L22Rik antibody
    • C6orf158 antibody
    • Inactive serine protease 35 antibody
    • MGC46520 antibody
    • P3D9 antibody
    • protease, serine, 35 antibody
    • PRSS 35 antibody
    see all

Anti-PRSS35 antibody images

  • Anti-PRSS35 antibody (ab89800) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PRSS35 antibody (ab89800)

ab89800 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"