Anti-PRUNE antibody (ab88613)
Key features and details
- Mouse polyclonal to PRUNE
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PRUNE antibody
See all PRUNE primary antibodies -
Description
Mouse polyclonal to PRUNE -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human PRUNE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Raw 264.7 cell lysate, NIH/3T3 cell lysate, and PRUNE transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. -
Tissue specificity
Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. -
Sequence similarities
Belongs to the PPase class C family. Prune subfamily. -
Cellular localization
Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed. - Information by UniProt
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Database links
- Entrez Gene: 58497 Human
- Entrez Gene: 229589 Mouse
- SwissProt: Q86TP1 Human
- SwissProt: Q8BIW1 Mouse
- Unigene: 728223 Human
- Unigene: 14155 Mouse
- Unigene: 279287 Mouse
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Alternative names
- DRES-17 antibody
- DRES17 antibody
- Drosophila-related expressed sequence 17 antibody
see all
Images
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Anti-PRUNE antibody (ab88613) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PRUNE antibody (ab88613) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PRUNE antibody (ab88613) at 1 µg/ml
Lane 1 : PRUNE transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab88613, at 10 µg/ml, staining PRUNE in HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88613 has been referenced in 2 publications.
- Santoro D et al. Evaluation of the expression and distribution of Prune-1 in the skin of healthy dogs. Vet Dermatol 30:446-447 (2019). PubMed: 31286578
- Zollo M et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). IF . PubMed: 28334956