Overview

  • Product name
    Anti-Prune homolog 2 antibody
  • Description
    Rabbit polyclonal to Prune homolog 2
  • Specificity
    Reacts specifically with human 299 kDa Prune homolog 2 protein
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from Cter domain of human Prune homolog 2.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/500.
ICC/IF 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 299 kDa.

Target

  • Relevance
    There are 2 named isoforms produced by alternative splicing.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • A214N16 3 antibody
    • BCH motif containing molecule at the carboxyl terminal region 1 antibody
    • BMCC1 antibody
    • BNIP2 motif containing molecule at the carboxyl terminal region 1 antibody
    • BNIPXL antibody
    • C9orf65 antibody
    • chromosome 9 open reading frame 65 antibody
    • prune homolog 2 (Drosophila) antibody
    • Prune2 antibody
    see all

Images

  • ICC/IF image of ab80262 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab80262 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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