Overview

  • Product name
    Anti-psbA antibody
  • Description
    Rabbit polyclonal to psbA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Tobacco
    Predicted to work with: Plants
  • Immunogen

    Synthetic peptide: (M)TAILERRESESLWGRFC, corresponding to N terminal amino acids 1-17 of Arabidopsis thaliana psbA

  • Positive control
    • Chloroplast (Arabidopsis and tobacco) and Synechocystis thylakoid proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 (for cyanobacteria) - 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight of precursor: 39 kDa).

    ab65579 may react in Synechocystis only with PsbA2/3 but not with PsbA1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      psbA, also known as D1, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. It reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.
    • Cellular localization
      Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein.
    • Database links
      • Alternative names
        • 32 kDa thylakoid membrane protein antibody
        • Photosystem II protein D1 antibody
        • Photosystem Q(B) protein antibody
        • psbA photosystem II protein D1 antibody

      Images

      • All lanes : Anti-psbA antibody (ab65579) at 1/2000 dilution

        Lane 1 : Arabidopsis chloroplast proteins
        Lane 2 : Tobacco chloroplast proteins
        Lane 3 : Synechocystis thylakoid proteins

        Developed using the ECL technique

        Predicted band size : 39 kDa
        Observed band size : 33 kDa (why is the actual band size different from the predicted?)

      References

      ab65579 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      We are very pleased to hear you would like to accept our offer and test ab65579 in barley. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forbarley and include this code in the ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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