Overview

  • Product nameAnti-psbA antibody
  • Description
    Rabbit polyclonal to psbA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana, Tobacco
    Predicted to work with: Plants
  • Immunogen

    Synthetic peptide: (M)TAILERRESESLWGRFC, corresponding to N terminal amino acids 1-17 of Arabidopsis thaliana psbA

  • Positive control
    • Chloroplast (Arabidopsis and tobacco) and Synechocystis thylakoid proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000 (for cyanobacteria) - 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight of precursor: 39 kDa).

    ab65579 may react in Synechocystis only with PsbA2/3 but not with PsbA1.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancepsbA, also known as D1, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. It reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.
    • Cellular localizationPlastid, chloroplast thylakoid membrane; Multi-pass membrane protein.
    • Database links
      • Alternative names
        • 32 kDa thylakoid membrane protein antibody
        • Photosystem II protein D1 antibody
        • Photosystem Q(B) protein antibody
        • psbA photosystem II protein D1 antibody

      Anti-psbA antibody images

      • All lanes : Anti-psbA antibody (ab65579) at 1/2000 dilution

        Lane 1 : Arabidopsis chloroplast proteins
        Lane 2 : Tobacco chloroplast proteins
        Lane 3 : Synechocystis thylakoid proteins

        Developed using the ECL technique

        Predicted band size : 39 kDa
        Observed band size : 33 kDa (why is the actual band size different from the predicted?)

      References for Anti-psbA antibody (ab65579)

      ab65579 has not yet been referenced specifically in any publications.

      Product Wall

      We are very pleased to hear you would like to accept our offer and test ab65579 in barley. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forbarley and include this code in the ...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"